Long and Short-Term Effect of mTOR Regulation on Cerebral Organoid Growth and Differentiations.

ABSTRACT:

Background

The mammalian target of rapamycin (mTOR) signaling is critical for the maintenance and differentiation of neurogenesis, and conceivably for many other brain developmental processes. However, in vivo studies of mTOR functions in the brain are often hampered due to the essential role of the associated signaling in brain development.

Methods

We monitored the long- and short-term effects of mTOR signaling regulation on cerebral organoids growth, differentiation and function using an mTOR inhibitor (everolimus) and an mTOR activator (MHY1485).

Results

Short-term treatment with MHY1485 induced faster organoid growth and differentiation, while long-term treatment induced the maturation of cerebral organoids.

Conclusion

These data suggest that the optimal ac

SUBMITTER: Park SB 

PROVIDER: S-EPMC10764682 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature