Estimating the Cholesterol Affinity of Integral Membrane Proteins from Experimental Data.

ABSTRACT: The cholesterol affinities of many integral plasma membrane proteins have been estimated by molecular computation. However, these values lack experimental confirmation. We therefore developed a simple mathematical model to extract sterol affinity constants and stoichiometries from published isotherms for the dependence of the activity of such proteins on the membrane cholesterol concentration. The binding curves for these proteins are sigmoidal, with strongly lagged thresholds attributable to competition for the cholesterol by bilayer phospholipids. The model provided isotherms that matched the experimental data using published values for the sterol association constants and stoichiometries of the phospholipids. Three oligomeric transporters were found to bind cholesterol without cooperati

SUBMITTER: Steck TL 

PROVIDER: S-EPMC10765374 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature