Base-Resolution Sequencing Methods for Whole-Transcriptome Quantification of mRNA Modifications.

ABSTRACT: ConspectusRNA molecules are not merely a combination of four bases of A, C, G, and U. Chemical modifications occur in almost all RNA species and play diverse roles in gene expression regulation. The abundant cellular RNAs, such as ribosomal RNA (rRNA) and transfer RNA (tRNA), are known to have the highest density of RNA modifications, which exert critical functions in rRNA and tRNA biogenesis, stability, and subsequent translation. In recent years, modifications on low-abundance RNA species in mammalian cells, such as messenger RNA (mRNA), regulatory noncoding RNA (ncRNA), and chromatin-associated RNA (caRNA), have been shown to contain multiple different chemical modifications with functional significance.As the most abundant mRNA modification in mammals, N⁶-methyladenos

SUBMITTER: Zhang LS 

PROVIDER: S-EPMC10765377 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature