RNA-mediated symmetry breaking enables singular olfactory receptor choice.

ABSTRACT: Olfactory receptor (OR) choice provides an extreme example of allelic competition for transcriptional dominance, where every olfactory neuron stably transcribes one of approximately 2,000 or more OR alleles^1,2. OR gene choice is mediated by a multichromosomal enhancer hub that activates transcription at a single OR^3,4, followed by OR-translation-dependent feedback that stabilizes this choice^5,6. Here, using single-cell genomics, we show formation of many competing hubs with variable enhancer composition, only one of which retains euchromatic features and transcriptional competence. Furthermore, we provide evidence that OR transcription recruits enhancers and reinforces enhancer hub activity locally, whereas OR RNA inhibits transcription of competing ORs ove

SUBMITTER: Pourmorady AD 

PROVIDER: S-EPMC10765522 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature