Acellular scaffold-based approach for in situ genetic engineering of host T-cells in solid tumor immunotherapy.

ABSTRACT:

Background

Targeted T-cell therapy has emerged as a promising strategy for the treatment of hematological malignancies. However, its application to solid tumors presents significant challenges due to the limited accessibility and heterogeneity. Localized delivery of tumor-specific T-cells using biomaterials has shown promise, however, procedures required for genetic modification and generation of a sufficient number of tumor-specific T-cells ex vivo remain major obstacles due to cost and time constraints.

Methods

Polyethylene glycol (PEG)-based three-dimensional (3D) scaffolds were developed and conjugated with positively charged poly-L-lysine (PLL) using carbamide chemistry for efficient loading of lentiviruses (LVs) carrying tumor antigen-specific T-cell receptors (TCRs).

SUBMITTER: Dandia HY 

PROVIDER: S-EPMC10765574 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature