Development and characterization of an immortalized swine respiratory cell line for influenza A virus research.

ABSTRACT:

Introduction

Swine serve as an important intermediate host species for generating novel influenza A viruses (IAVs) with pandemic potential because of the host's susceptibility to IAVs of swine, human and avian origin. Primary respiratory cell lines are used in IAV research to model the host's upper respiratory tract in vitro. However, primary cell lines are limited by their passaging capacity and are time-consuming for use in industry and research pipelines. We were interested in developing and characterizing a biologically relevant immortalized swine respiratory cell line that could be used for efficient propagation and characterization of swine IAV isolates.

Methods

Lung tissue for the generation of primary swine respiratory cells were isolated from the bronchi of a

SUBMITTER: Neasham PJ 

PROVIDER: S-EPMC10765598 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature