IPSC-derived PSEN2 (N141I) astrocytes and microglia exhibit a primed inflammatory phenotype.

ABSTRACT:

Background

Widescale evidence points to the involvement of glia and immune pathways in the progression of Alzheimer's disease (AD). AD-associated iPSC-derived glial cells show a diverse range of AD-related phenotypic states encompassing cytokine/chemokine release, phagocytosis and morphological profiles, but to date studies are limited to cells derived from PSEN1, APOE and APP mutations or sporadic patients. The aim of the current study was to successfully differentiate iPSC-derived microglia and astrocytes from patients harbouring an AD-causative PSEN2 (N141I) mutation and characterise the inflammatory and morphological profile of these cells.

Methods

iPSCs from three healthy control individuals and three familial AD patients harbouring a heterozygous PSEN2 (N141I) mutation

SUBMITTER: Sullivan MA 

PROVIDER: S-EPMC10765839 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature