PRMT2 silencing regulates macrophage polarization through activation of STAT1 or inhibition of STAT6.

ABSTRACT:

Background

Macrophages play significant roles in innate immune responses and are heterogeneous cells that can be polarized into M1 or M2 phenotypes. PRMT2 is one of the type I protein arginine methyltransferases involved in inflammation. However, the role of PRMT2 in M1/M2 macrophage polarization remains unclear. Our study revealed the effect and mechanism of PRMT2 in macrophage polarization.

Methods

Bone marrow-derived macrophages (BMDMs) were polarized to M1 or M2 state by LPS plus murine recombinant interferon-γ (IFN-γ) or interleukin-4 (IL-4). Quantitative polymerase chain reaction (qPCR), western blot and flow cytometry (FCM) assay were performed and analyzed markers and signaling pathways of macrophage polarization.

Results

We found that PRMT2 was obviously upre

SUBMITTER: Liu T 

PROVIDER: S-EPMC10765854 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature