MYH1G-AS is a chromatin-associated lncRNA that regulates skeletal muscle development in chicken.

ABSTRACT:

Background

Skeletal muscle development is pivotal for animal growth and health. Recently, long noncoding RNAs (lncRNAs) were found to interact with chromatin through diverse roles. However, little is known about how lncRNAs act as chromatin-associated RNAs to regulate skeletal muscle development. Here, we aim to investigate the regulation of chromatin-associated RNA (MYH1G-AS) during skeletal muscle development.

Methods

We provided comprehensive insight into the RNA profile and chromatin accessibility of different myofibers, combining RNA sequencing (RNA-seq) with an assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq). The dual-luciferase reporter assay and chromatin immunoprecipitation (ChIP) assay were used to analyze the transcriptional r

SUBMITTER: Cai B 

PROVIDER: S-EPMC10765903 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature