TET2-mediated ECM1 hypomethylation promotes the neovascularization in active proliferative diabetic retinopathy.

ABSTRACT:

Background

Studies have shown that tet methylcytosine dioxygenase 2 (TET2) is highly expressed in diabetic retinopathy (DR), which reduces the DNA methylation of downstream gene promoters and activates the transcription. Abnormally expressed TET2 and downstream genes in a high-glucose environment are associated with retinal capillary leakage and neovascularization. Here, we investigated the downstream genes of TET2 and its potential association with neovascularization in proliferative diabetic retinopathy (PDR).

Methods

GSE60436, GSE57362, and GSE158333 datasets were analyzed to identify TET2-related hypomethylated and upregulated genes in PDR. Gene expression and promoter methylation of these genes under high glucose treatment were verified. Moreover, TET2 knockdown was use

SUBMITTER: Cai C 

PROVIDER: S-EPMC10765922 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature