Induction of CD8+ Immune Memory and Enhanced Inflammation in a Skin Inflammation Model through Pre-Immunization with Inactivated Pathogens.

ABSTRACT: Laboratory mice live in specific pathogen-free (SPF) conditions, resulting in an immature immune system comparable to that of new-borns rather than adult humans or mice from pet shops. This condition may compromise their translational value. Reintroducing pathogens would lead to the uncontrolled spread of infections and associated diseases, so research facilities should seek safer alternatives. We immunized laboratory mice with a cocktail of pathogens, which were inactivated by ultraviolet irradiation and mixed with the adjuvant Addavax. This immunization resulted in a higher percentage of CD8+ effector memory T cells compared to untreated mice, although the response was not as robust as in pet shop mice. In a model of skin inflammation, pre-immunization led to an increased skin inflammato

SUBMITTER: Falkenberg C 

PROVIDER: S-EPMC10766028 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature