MiR-9 promotes canine endothelial-like cell migration by targeting COL15A1.

ABSTRACT:

Background

Endothelial cell migration is the initial stage of angiogenesis. In previous studies, miR-9 has been found to regulate angiogenesis and cell migration in human medicine.

Objectives

This study aimed to reveal the regulatory effect of miR-9 on canine endothelial cell migration.

Methods

Embryonic canine ventricle myocardium tissues were collected and induced to differentiate into endothelial-like cells (ELCs). A transwell and invasion assay were used to evaluate the impact of miR-9 on the migration capacity of ELCs, after which a luciferase reporter assay, western blotting, RNA sequencing and reverse transcription-polymerase chain reaction were conducted to explore the regulatory mechanism.

Results

Our results showed that we successfully induced the pri

SUBMITTER: Jiang H 

PROVIDER: S-EPMC10766037 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature