Differential transcriptome response of blood brain barrier spheroids to neuroinvasive Neisseria and Borrelia.

ABSTRACT:

Background

The blood-brain barrier (BBB), a highly regulated interface between the blood and the brain, prevents blood-borne substances and pathogens from entering the CNS. Nevertheless, pathogens like Neisseria meningitidis and Borrelia bavariensis can breach the BBB and infect the brain parenchyma. The self-assembling BBB-spheroids can simulate the cross talk occurring between the cells of the barrier and neuroinvasive pathogens.

Methods

BBB spheroids were generated by co-culturing human brain microvascular endothelial cells (hBMECs), pericytes and astrocytes. The BBB attributes of spheroids were confirmed by mapping the localization of cells, observing permeability of angiopep2 and non-permeability of dextran. Fluorescent Neisseria, Borrelia or

SUBMITTER: Kulkarni A 

PROVIDER: S-EPMC10766361 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature