Identification and functional prediction of long non-coding RNAs related to oxidative stress in the jejunum of piglets.

ABSTRACT:

Objective

Oxidative stress (OS) is a pathological process arising from the excessive production of free radicals in the body. It has the potential to alter animal gene expression and cause damage to the jejunum. However, there have been few reports of changes in the expression of long noncoding RNAs (lncRNAs) in the jejunum in piglets under OS. The purpose of this research was to examine how lncRNAs in piglet jejunum change under OS.

Methods

The abdominal cavities of piglets were injected with diquat (DQ) to produce OS. Raw reads were downloaded from the SRA database. RNA-seq was utilized to study the expression of lncRNAs in piglets under OS. Additionally, six randomly selected lncRNAs were verified using quantitative real-time polymerase chain reaction (qRT‒PCR) to examine

SUBMITTER: Li J 

PROVIDER: S-EPMC10766486 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature