In-house validation of an LC-MS method for the multiplexed quantitative determination of total allergenic food in chocolate.

ABSTRACT: Mass spectrometry has been widely accepted as a confirmatory tool for the sensitive detection of undeclared presence of allergenic ingredients. Multiple methods have been developed so far, achieving different levels of sensitivity and robustness, still lacking harmonization of the analytical validation and impairing comparability of results. In this investigation, a quantitative method has been validated in-house for the determination of six allergenic ingredients (cow's milk, hen's egg, peanut, soybean, hazelnut, and almond) in a chocolate-based matrix. The latter has been produced in a food pilot plant to provide a real and well-characterized matrix for proper assessment of method performance characteristics according to official guidelines. In particular, recent considerations issued by

SUBMITTER: Pilolli R 

PROVIDER: S-EPMC10766722 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature