Schistosoma mansoni vaccine candidates identified by unbiased phage display screening in self-cured rhesus macaques.

ABSTRACT: Schistosomiasis, a challenging neglected tropical disease, affects millions of people worldwide. Developing a prophylactic vaccine against Schistosoma mansoni has been hindered by the parasite's biological complexity. In this study, we utilized the innovative phage-display immunoprecipitation followed by a sequencing approach (PhIP-Seq) to screen the immune response of 10 infected rhesus macaques during self-cure and challenge-resistant phases, identifying vaccine candidates. Our high-throughput S. mansoni synthetic DNA phage-display library encoded 99.6% of 119,747 58-mer peptides, providing comprehensive coverage of the parasite's proteome. Library screening with rhesus macaques' antibodies, from the early phase of establishment of parasite infection, identified significantly enriched ep

SUBMITTER: Woellner-Santos D 

PROVIDER: S-EPMC10767053 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature