Applying targeted gene hybridization capture to viruses with a focus to SARS-CoV-2.

ABSTRACT: Although next-generation sequencing technologies are advancing rapidly, many research topics often require selective sequencing of genomic regions of interest. In addition, sequencing low-titre viruses is challenging, especially for coronaviruses, which are the largest RNA viruses. Prior to sequencing, enrichment of viral particles can help to significantly increase target sequence information as well as avoid large sequencing efforts and, consequently, can increase sensitivity and reduce sequencing costs. Targeting nucleic acids using capture by hybridization is another efficient method that can be performed by applying complementary probes (DNA or RNA baits) to directly enrich genetic information of interest while removing background non-target material. In studies where sequence capture

SUBMITTER: Ceballos-Garzon A 

PROVIDER: S-EPMC10767490 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature