Tuning spacer length improves the functionality of the nanobody-based VEGFR2 CAR T cell.

ABSTRACT:

Background

The chimeric antigen receptor-expressing T (CAR-T) cells for cancer immunotherapy have obtained considerable clinical importance. CAR T cells need an optimized intracellular signaling domain to get appropriately activated and also for the proper antigen recognition, the length and composition of the extracellular spacer are critical factors.

Results

We constructed two third-generation nanobody-based VEGFR2-CARs containing either IgG1 hinge-CH2-CH3 region or hinge-only as long or short extracellular spacers, respectively. Both CARs also contained intracellular activating domains of CD28, OX40, and CD3ζ. The T cells from healthy individuals were transduced efficiently with the two CARs, and showed increased secretion of IL-2 and IFN-γ cytokines, and also CD69 and CD

SUBMITTER: Taheri FH 

PROVIDER: S-EPMC10768260 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature