Application of a recombinant novel trypsin from Trichinella spiralis for serodiagnosis of trichinellosis.

ABSTRACT:

Background

The excretory/secretory (ES) antigen of Trichinella spiralis muscle larvae (ML) is currently the most widely used diagnostic antigen to detect T. spiralis infection. However, this antigen has certain drawbacks, such as a complicated ES antigen preparation process and lower sensitivity during the early phase of infection. The aim of this study was to investigate the features of a novel T. spiralis trypsin (TsTryp) and evaluate its potential diagnostic value for trichinellosis.

Methods

The TsTryp gene was cloned and recombinant TsTryp (rTsTryp) expressed. Western blotting and an enzyme-linked immunosorbent assay (ELISA) were performed to confirm the antigenicity of rTsTryp. The expression pattern and distribution signature of TsTryp at various life-cycle stages of T

SUBMITTER: Han LL 

PROVIDER: S-EPMC10768479 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature