Spectral Analysis of Human Retinal Pigment Epithelium Cells in Healthy and AMD Eyes.

ABSTRACT:

Purpose

Retinal pigment epithelium (RPE) cells show strong autofluorescence (AF). Here, we characterize the AF spectra of individual RPE cells in healthy eyes and those affected by age-related macular degeneration (AMD) and investigate associations between AF spectral response and the number of intracellular AF granules per cell.

Methods

RPE-Bruch's membrane flatmounts of 22 human donor eyes, including seven AMD-affected eyes (early AMD, three; geographic atrophy, one; neovascular, three) and 15 unaffected macula (<51 years, eight; >80 years, seven), were imaged at the fovea, perifovea, and near-periphery using confocal AF microscopy (excitation 488 nm), and emission spectra were recorded (500-710 nm). RPE cells were manually segmented with computer assistance and stratified

SUBMITTER: Bourauel L 

PROVIDER: S-EPMC10768704 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature