Cerebrospinal fluid CD14⁺⁺CD16⁺ monocytes in HIV-1 subtype C compared with subtype B.

ABSTRACT: CD14⁺⁺CD16⁺ monocytes are susceptible to HIV-1 infection, and cross the blood-brain barrier. HIV-1 subtype C (HIV-1C) shows reduced Tat protein chemoattractant activity compared to HIV-1B, which might influence monocyte trafficking into the CNS. We hypothesized that the proportion of monocytes in CSF in HIV-1C is lower than HIV-1B group. We sought to assess differences in monocyte proportions in cerebrospinal fluid (CSF) and peripheral blood (PB) between people with HIV (PWH) and without HIV (PWoH), and by HIV-1B and -C subtypes. Immunophenotyping was performed by flow cytometry, monocytes were analyzed within CD45 + and CD64 + gated regions and classified in classical (CD14⁺⁺CD16^-), intermediate (CD14⁺⁺CD16⁺), and non-class

SUBMITTER: de Almeida SM 

PROVIDER: S-EPMC10769008 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature