EIF5A controls mitoprotein import by relieving ribosome stalling at the TIM50 translocase mRNA.

ABSTRACT: The efficient import of nuclear-encoded proteins into mitochondria is crucial for proper mitochondrial function. The conserved translation factor eIF5A is primarily known as an elongation factor which binds ribosomes to alleviate ribosome stalling at sequences encoding polyprolines or combinations of proline with glycine and charged amino acids. eIF5A is known to impact the mitochondrial function across a variety of species although the precise molecular mechanism underlying this impact remains unclear. We found that depletion of eIF5A in yeast drives reduced translation and levels of TCA cycle and oxidative phosphorylation proteins. We further found that loss of eIF5A leads to the accumulation of mitoprotein precursors in the cytosol as well as to the induction of a mitochondrial import s

SUBMITTER: Barba-Aliaga M 

PROVIDER: S-EPMC10769225 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature