The promise and peril of comparing fluorescence lifetime in biology revealed by simulations.

ABSTRACT: Signaling dynamics are crucial in biological systems, and biosensor-based real-time imaging has revolutionized their analysis. Fluorescence lifetime imaging microscopy (FLIM) excels over the widely used fluorescence intensity imaging by allowing the measurement of absolute signal levels independent of sensor concentration. This capability enables the comparison of signaling dynamics across different animals, body regions, and timeframes. However, FLIM's advantage can be compromised by factors like autofluorescence in biological experiments. To address this, we introduce FLiSimBA, a flexible computational framework for realistic Fluorescence Lifetime Simulation for Biological Applications. Through simulations, we analyze the signal-to-noise ratios of fluorescence lifetime data, determining

SUBMITTER: Ma P 

PROVIDER: S-EPMC10769356 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature