STOPGAP, an open-source package for template matching, subtomogram alignment, and classification.

ABSTRACT: Cryo-electron tomography (cryo-ET) enables molecular-resolution 3D imaging of complex biological specimens such as viral particles, cellular sections, and in some cases, whole cells. This enables the structural characterization of molecules in their near-native environments, without the need for purification or separation, thereby preserving biological information such as conformational states and spatial relationships between different molecular species. Subtomogram averaging is an image processing workflow that allows users to leverage cryo-ET data to identify and localize target molecules, determine high-resolution structures of repeating molecular species, and classifying different conformational states. Here we describe STOPGAP, an open-source package for subtomogram averaging designe

SUBMITTER: Wan W 

PROVIDER: S-EPMC10769363 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature