Chromosome architecture in an archaeal species naturally lacking structural maintenance of chromosomes proteins.

ABSTRACT: Proteins in the structural maintenance of chromosomes (SMC) superfamily play key roles in chromosome organization and are ubiquitous across all domains of life. However, SMC proteins are notably absent in the Desulfurococcales of phylum Crenarchaeota. Intrigued by this observation, we performed chromosome conformation capture experiments in the model Desulfurococcales species Aeropyrum pernix. As in other archaea, we observe chromosomal interaction domains across the chromosome. The boundaries between chromosomal interaction domains show a dependence on transcription and translation for their definition. Importantly, however, we reveal an additional higher-order, bipartite organization of the chromosome-with a small high-gene-expression and self-interacting domain that is defined by transc

SUBMITTER: Badel C 

PROVIDER: S-EPMC10769869 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature