Application of real-time PCR for the identification of the endangered species Galemys pyrenaicus through faecal samples.

ABSTRACT:

Background

Currently, many micromammals are important targets for study. The endangered Galemys pyrenaicus is an outstanding example. Globally, their populations have suffered a substantial decline in last 20 years. In the surveyed area, the capture of desman is legally forbidden due to the high conservation concerns. Reason by non-invasive sampling through faeces is proposed for its monitoring. Furthermore, the confusion between faeces from desman and Mediterranean water shrews must be considered. Thus, the aim of this study was focused on developing RT-PCR assays to determine the presence of Galemys pyrenaicus and N. a. anomalus from non-invasive samples.

Methods and results

The study was conducted in the mountains of the System Central of Extremadura (Spain). A total of 1

SUBMITTER: Ripa A 

PROVIDER: S-EPMC10770224 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature