Paneth-like cells produced from OLFM4⁺ stem cells support OLFM4⁺ stem cell growth in advanced colorectal cancer.

ABSTRACT: Tumor tissues consist of heterogeneous cells that originate from stem cells; however, their cell fate determination program remains incompletely understood. Using patient-derived organoids established from patients with advanced colorectal cancer (CRC), we evaluated the potential of olfactomedin 4 (OLFM4)⁺ stem cells to produce a bifurcated lineage of progenies with absorptive and secretory properties. In the early phases of organoid reconstruction, OLFM4⁺ cells preferentially gave rise to secretory cells. Additionally, we found that Paneth-like cells, which do not exist in the normal colon, were induced in response to Notch signaling inhibition. Video recordings of single OLFM4⁺ cells revealed that organoids containing Paneth-like cells were effectively pr

SUBMITTER: Sakahara M 

PROVIDER: S-EPMC10770338 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature