Centriole and transition zone structures in photoreceptor cilia revealed by cryo-electron tomography.

ABSTRACT: Primary cilia mediate sensory signaling in multiple organisms and cell types but have structures adapted for specific roles. Structural defects in them lead to devastating diseases known as ciliopathies in humans. Key to their functions are structures at their base: the basal body, the transition zone, the "Y-shaped links," and the "ciliary necklace." We have used cryo-electron tomography with subtomogram averaging and conventional transmission electron microscopy to elucidate the structures associated with the basal region of the "connecting cilia" of rod outer segments in mouse retina. The longitudinal variations in microtubule (MT) structures and the lumenal scaffold complexes connecting them have been determined, as well as membrane-associated transition zone structures: Y-shaped links

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC10770417 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature