Septic macrophages induce T cells immunosuppression in a cell-cell contact manner with the involvement of CR3.

ABSTRACT:

Background

In addition to excessive inflammation, immunosuppression has been recognized as a contributing factor to poor prognosis of sepsis. Although it has been reported that T cells can become functionally impaired during sepsis, the underlying mechanisms responsible for this phenomenon remain unclear. This study aims to elucidate the mechanisms by which macrophages induce immunosuppression in T cells.

Methods

In an in vivo setting, C57BL-6J mice were subjected to cecal ligation and puncture (CLP) with or without depletion of macrophages, and the functions of T cells were assessed. In vitro experiments involved direct co-culture or separate culture of T cells and septic macrophages using a transwell system, followed by analysis of T cell immunity. Additionally, a siRNA ta

SUBMITTER: Huang S 

PROVIDER: S-EPMC10770445 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature