The E156K mutation in the CRYAA gene affects the epithelial-mesenchymal transition and migration of human lens epithelial cells.

ABSTRACT:

Purpose

To investigated the biological effects of E156K-mutated αA-crystallin (CRYAA) in human lens epithelial cells (HLECs).

Methods

FLAG-tagged, human, full-length, wild-type (WT), or E156K-mutated CRYAA was expressed in HLECs under CRYAA knockdown. CRYAA expression was determined by quantitative reverse transcription polymerase chain reaction and western blotting (WB). Rhodamine cytoskeleton staining was used to observe the changes in cell morphology following transfection with WT or E156K-mutated CRYAA plasmids. WB was performed to assess the expression of markers related to epithelial-mesenchymal transition (EMT) and migration.

Results

Rhodamine cytoskeleton staining revealed changes in the morphology of cells transfected with E156K-mutated CRYAA

SUBMITTER: Zhao Z 

PROVIDER: S-EPMC10770500 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature