Digital analysis of the prostate tumor microenvironment with high-order chromogenic multiplexing.

ABSTRACT: As our understanding of the tumor microenvironment grows, the pathology field is increasingly utilizing multianalyte diagnostic assays to understand important characteristics of tumor growth. In clinical settings, brightfield chromogenic assays represent the gold-standard and have developed significant trust as the first-line diagnostic method. However, conventional brightfield tests have been limited to low-order assays that are visually interrogated. We have developed a hybrid method of brightfield chromogenic multiplexing that overcomes these limitations and enables high-order multiplex assays. However, how compatible high-order brightfield multiplexed images are with advanced analytical algorithms has not been extensively evaluated. In the present study, we address this gap by developi

SUBMITTER: Rajendran R 

PROVIDER: S-EPMC10770522 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature