High species homology potentiates quantitative inflammation profiling in zebrafish using immunofluorescence.

ABSTRACT: Due to substantial homology between the human and zebrafish genome and a high level of conservation of the innate immune system across species, zebrafish larvae have become an invaluable research tool for studying inflammation and modelling inflammatory disease. However, further microscopy techniques need to be developed for better profiling of inflammation and in particular, integrated cytokine responses to different stimuli - approaches are currently largely limited to assessment of changes in cytokine gene transcription and in vivo visualisation using transgenics, which is limited in terms of the number of cytokines that may be assessed at once. In this study, after confirming substantial homology of human vs zebrafish cytokine amino acid sequences, immunofluorescence staining us

SUBMITTER: Ollewagen T 

PROVIDER: S-EPMC10770569 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature