Sialic acid-modified der p 2 allergen exerts immunomodulatory effects on human PBMCs.

ABSTRACT:

Background

House dust mite extract-based allergen immunotherapy (AIT) to treat house dust mite allergy is substantially effective but still presents some safety and efficacy concerns that warrant improvement. Several major allergen-based approaches to increase safety and efficacy of AIT have been proposed. One of them is the use of the group 2 allergen, Der p 2.

Objective

We sought to investigate the immunomodulatory effects of sialic acid-modified major allergen recombinant Der p 2 (sia-rDer p 2) on PBMCs from healthy volunteers.

Methods

We activated PBMCs with anti-CD3/CD28 antibodies and incubated them at 37°C for 6 days in the presence or absence of either native rDer p 2 or α2-3 sialic acid-modified rDer p 2 (sia-rDer p 2). We assessed the changes in CD4⁺

SUBMITTER: Keumatio Doungtsop BC 

PROVIDER: S-EPMC10770723 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature