Probing the interaction of lysozyme with cardiac glycoside digitoxin: experimental and in silico analyses.

ABSTRACT: Digitoxin is a cardiac glycoside used to treat heart failure and heart arrhythmia. However, its therapeutic concentration range is very narrow. High doses of digitoxin are associated with severe side effects; therefore, it is necessary to develop the delivery system which can control the plasma levels of it. In this context, the binding of lysozyme, an important protein having many applications, with digitoxin has been studied to see the ability of the former as a carrier. The studies were carried out using both experimental and computational methods. The intrinsic fluorescence of lysozyme increased on the addition of digitoxin. Fluorescence results suggested that there was a strong interaction between lysozyme and digitoxin which was favored, mainly, by hydrophobic forces. Further, digito

SUBMITTER: Ali MS 

PROVIDER: S-EPMC10770834 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature