WRN loss accelerates abnormal adipocyte metabolism in Werner syndrome.

ABSTRACT:

Background

Metabolic dysfunction is one of the main symptoms of Werner syndrome (WS); however, the underlying mechanisms remain unclear. Here, we report that loss of WRN accelerates adipogenesis at an early stage both in vitro (stem cells) and in vivo (zebrafish). Moreover, WRN depletion causes a transient upregulation of late-stage of adipocyte-specific genes at an early stage.

Methods

In an in vivo study, we generated wrn^-/- mutant zebrafish and performed histological stain and Oil Red O staining to assess the fat metabolism. In an in vitro study, we used RNA-seq and ATAC-seq to profile the transcriptional features and chromatin accessibility in WRN depleted adipocytes. Moreover, we performed ChIP-seq to further study the regulatory mechanisms of metabolic dysfu

SUBMITTER: Tian Y 

PROVIDER: S-EPMC10770995 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature