In vitro induction of hair follicle signatures using human dermal papilla cells encapsulated in fibrin microgels.

ABSTRACT: Cellular spheroids have been described as an appropriate culture system to restore human follicle dermal papilla cells (hFDPc) intrinsic properties; however, they show a low and variable efficiency to promote complete hair follicle formation in in vivo experiments. In this work, a conscientious analysis revealed a 25% cell viability in the surface of the dermal papilla spheroid (DPS) for all culture conditions, questioning whether it is an appropriate culture system for hFDPc. To overcome this problem, we propose the use of human blood plasma for the generation of fibrin microgels (FM) with encapsulated hFDPc to restore its inductive signature, either in the presence or in the absence of blood platelets. FM showed a morphology and extracellular matrix composition similar to the native derm

SUBMITTER: Quilez C 

PROVIDER: S-EPMC10771113 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature