Exploring a general multi-pronged activation strategy for natural product discovery in Actinomycetes.

ABSTRACT: Natural products possess significant therapeutic potential but remain underutilized despite advances in genomics and bioinformatics. While there are approaches to activate and upregulate natural product biosynthesis in both native and heterologous microbial strains, a comprehensive strategy to elicit production of natural products as well as a generalizable and efficient method to interrogate diverse native strains collection, remains lacking. Here, we explore a flexible and robust integrase-mediated multi-pronged activation approach to reliably perturb and globally trigger antibiotics production in actinobacteria. Across 54 actinobacterial strains, our approach yielded 124 distinct activator-strain combinations which consistently outperform wild type. Our approach expands accessible metab

SUBMITTER: Tay DWP 

PROVIDER: S-EPMC10771470 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature