Two-photon excitation fluorescence microspectroscopy protocols for examining fluorophores in fossil plants.

ABSTRACT: Fluorescence emission is common in plants. While fluorescence microscopy has been widely used to study living plants, its application in quantifying the fluorescence of fossil plants has been limited. Fossil plant fluorescence, from original fluorophores or formed during fossilization, can offer valuable insights into fluorescence in ancient plants and fossilization processes. In this work, we utilize two-photon fluorescence microspectroscopy to spatially and spectrally resolve the fluorescence emitted by amber-embedded plants, leaf compressions, and silicified wood. The advanced micro-spectroscope utilized, with its pixel-level spectral resolution and line-scan excitation capabilities, allows us to collect comprehensive excitation and emission spectra with high sensitivity and minimal las

SUBMITTER: Stoneman MR 

PROVIDER: S-EPMC10771488 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature