Rapid, label-free enrichment of lymphocytes in a closed system using a flow-through microfluidic device.

ABSTRACT: The majority of adoptive cellular therapies are produced from peripheral mononuclear cells obtained via leukapheresis and further enriched for the cells of interest (e.g., T cells). Here, we present a first-of-its-kind closed system, which effectively removes ~85% of monocytes and ~88% of platelets, while recovering ~88% of concentrated T cells in a separate output stream, as the leukapheresis sample flows through a microfluidic device at 5 mL/min. The system is driven by a common peristaltic pump, enabled by a novel pressure wave dampener, and operates in a closed bag-to-bag configuration, without requiring any specialized, dedicated equipment. When compared to standard density gradient centrifugation on paired samples, the new system demonstrated a 1.5-fold increase in T cell recovery an

SUBMITTER: Mukhamedshin A 

PROVIDER: S-EPMC10771558 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature