Establishment of a multiplex PCR-CE assay for the simultaneous and rapid analysis of age markers for Calliphora vicina pupae.

ABSTRACT: Insects, especially blow flies, are forensically relevant to determine the minimal postmortem interval (PMI_min), based on the fact that they are usually the first colonisers of a body. By estimating the age of immature blow flies, interferences can be made about the time since death. Whilst morphological parameters are valuable for age estimation of blow fly larvae, gene expression profiling is more applicable for blow fly pupae. Here, the age-dependent changes in the gene expression levels during the development are analysed. 28 temperature-independent markers have already been described for the age estimation of pupae of the forensically important blow fly Calliphora vicina and are analysed by RT-qPCR. To allow simultaneous analysis of these age markers, a multiplex assay was

SUBMITTER: Hartmann K 

PROVIDER: S-EPMC10771996 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature