A two-component signal transduction system essential for growth of Bacillus subtilis: implications for anti-infective therapy.

ABSTRACT: A two-component signal transduction system encoded by the yycF and yycG genes is part of an operon containing three genes, yycH, yycI, and yycJ, with no known function and a gene, yycK, coding for an HtrA-like protease. This operon was transcribed during growth, and its transcription shut down as the cells approached stationary phase. This decreased transcription was not Spo0A dependent. The HtrA protease gene was separately controlled during sporulation from a sigmaG promoter. Studies using insertional inactivation plasmids revealed that neither yycF nor yycG could be inactivated, whereas the other genes were inactivated without loss of viability. A temperature-sensitive YycF response regulator mutant was isolated and shown to have an H215P mutation in a putative DNA-binding domain which

SUBMITTER: Fabret C 

PROVIDER: S-EPMC107725 | biostudies-literature | 1998 Dec

REPOSITORIES: biostudies-literature