Reassessment of dextran sulfate in anti-Xa assay for unfractionated heparin laboratory monitoring.

ABSTRACT:

Background

Anti-Xa assays are used for unfractionated heparin (UFH) monitoring. Dextran sulfate (DS) is used in some assays to overcome the artifactual preanalytical release of platelet factor 4. However, the practical implications of this test modification have not been studied extensively.

Objectives

To investigate the impact of the presence of DS in the anti-Xa assay for UFH laboratory monitoring.

Methods

We studied factor Xa inhibition, using an assay without DS (Stago Liquid Anti-Xa), in normal pool plasma spiked with various concentrations of UFH (up to 1 IU/mL) in the presence of increasing concentrations of DS (up to 2560 μg/mL). We also investigated the effect of DS on FXa inhibition measured after the addition of UFH and heparin antagonists (protamine and Po

SUBMITTER: Hardy M 

PROVIDER: S-EPMC10772882 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature