Prunella vulgaris polysaccharide inhibits herpes simplex virus infection by blocking TLR-mediated NF-κB activation.

ABSTRACT:

Background

Prunella vulgaris polysaccharide extracted by hot water and 30% ethanol precipitation (PVE30) was reported to possess potent antiviral effects against herpes simplex virus (HSV) infection. However, its anti-HSV mechanism has not yet been fully elucidated.

Purpose

This study aimed to investigate the potential mechanisms of PVE30 against HSV infection.

Methods

Antiviral activity was evaluated by a plaque reduction assay, and the EC₅₀ value was calculated. Immunofluorescence staining and heparin bead pull-down assays confirmed the interactions between PVE30 and viral glycoproteins. Real-time PCR was conducted to determine the mRNA levels of viral genes, including UL54, UL29, UL27, UL44, and US6, and the proinflammatory cytokines IL-6 and TNF-α. The

SUBMITTER: Zhong X 

PROVIDER: S-EPMC10773030 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature