Comparison of capture-based mtDNA sequencing performance between MGI and illumina sequencing platforms in various sample types.

ABSTRACT:

Background

Mitochondrial genome abnormalities can lead to mitochondrial dysfunction, which in turn affects cellular biology and is closely associated with the development of various diseases. The demand for mitochondrial DNA (mtDNA) sequencing has been increasing, and Illumina and MGI are two commonly used sequencing platforms for capture-based mtDNA sequencing. However, there is currently no systematic comparison of mtDNA sequencing performance between these two platforms. To address this gap, we compared the performance of capture-based mtDNA sequencing between Illumina's NovaSeq 6000 and MGI's DNBSEQ-T7 using tissue, peripheral blood mononuclear cell (PBMC), formalin-fixed paraffin-embedded (FFPE) tissue, plasma, and urine samples.

Results

Our analysis indicated a high de

SUBMITTER: Feng Z 

PROVIDER: S-EPMC10773069 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature