The P2Y₁ receptor in the colonic myenteric plexus of rats and its correlation with opioid-induced constipation.

ABSTRACT: This study was designed to explore the expression changes of P2Y₁ receptors in the distal colonic myenteric layer of rats. An opioid induced constipation(OIC) rat model was generated by intraperitoneal (i.p) injection of loperamide. At 7 days post-treatment, the model rats were assessed by calculating the fecal water content and the gastrointestinal transit ratio. The immunofluorescence (IF)-based histochemical study was used to observe the distribution of P2Y₁ receptors in the distal colonic myenteric plexus. Western blotting (WB) was performed to evaluate the expression changes of P2Y₁ proteins in the myenteric layer, and the electrophysiological approaches were carried out to determine the regulatory roles of P2Y₁ receptors on distal colonic m

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC10773096 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature