Improved biosensing of Legionella by integrating filtration and immunomagnetic separation of the bacteria retained in filters.

ABSTRACT: A novel approach is presented that combines filtration and the direct immunomagnetic separation of the retained bacteria Legionella in filters, for further electrochemical immunosensing. This strategy allows for the separation and preconcentration of the water-borne pathogen from high-volume samples, up to 1000 mL. The limit of detection of the electrochemical immunosensor resulted in 100 CFU mL^-1 and improved up to 0.1 CFU mL^-1 when the preconcentration strategy was applied in 1 L of sample (10³-fold improvement). Remarkably, the immunosensor achieves the limit of detection in less than 2.5 h and simplified the analytical procedure. This represents the lowest concentration reported to date for electrochemical immunosensing of Legionella cells without the n

SUBMITTER: Mesas Gomez M 

PROVIDER: S-EPMC10774190 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature