Unknown

Dataset Information

0

Endolysin EN572-5 as an alternative to treat urinary tract infection caused by Streptococcus agalactiae.


ABSTRACT: Streptococcus agalactiae (Group B Streptococcus, GBS) is an opportunistic pathogen causing urinary tract infection (UTI). Endolysin EN572-5 was identified in prophage KMB-572-E of the human isolate Streptococcus agalactiae KMB-572. The entire EN572-5 gene was cloned into an expression vector and the corresponding recombinant protein EN572-5 was expressed in Escherichia coli in a soluble form, isolated by affinity chromatography, and characterized. The isolated protein was highly active after 30 min incubation in a temperature range of - 20 °C to 37 °C and in a pH range of 5.5-8.0. The endolysin EN572-5 lytic activity was tested on different Streptococcus spp. and Lactobacillus spp. The enzyme lysed clinical GBS (n = 31/31) and different streptococci (n = 6/8), and also exhibited moderate lytic activity against UPEC (n = 4/4), but no lysis of beneficial vaginal lactobacilli (n = 4) was observed. The ability of EN572-5 to eliminate GBS during UTI was investigated using an in vitro model of UPSA. After the administration of 3 μM EN572-5, a nearly 3-log decrease of urine bacterial burden was detected within 3 h. To date, no studies have been published on the use of endolysins against S. agalactiae during UTI. KEY POINTS: • A lytic protein, EN572-5, from a prophage of a human GBS isolate has been identified. • This protein is easily produced, simple to prepare, and stable after lyophilization. • The bacteriolytic activity of EN572-5 was demonstrated for the first time in human urine.

SUBMITTER: Kajsikova M 

PROVIDER: S-EPMC10774192 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Endolysin EN572-5 as an alternative to treat urinary tract infection caused by Streptococcus agalactiae.

Kajsikova Maria M   Kajsik Michal M   Bocanova Lucia L   Papayova Kristina K   Drahovska Hana H   Bukovska Gabriela G  

Applied microbiology and biotechnology 20240108 1


Streptococcus agalactiae (Group B Streptococcus, GBS) is an opportunistic pathogen causing urinary tract infection (UTI). Endolysin EN572-5 was identified in prophage KMB-572-E of the human isolate Streptococcus agalactiae KMB-572. The entire EN572-5 gene was cloned into an expression vector and the corresponding recombinant protein EN572-5 was expressed in Escherichia coli in a soluble form, isolated by affinity chromatography, and characterized. The isolated protein was highly active after 30  ...[more]

Similar Datasets

| S-EPMC6455028 | biostudies-literature
| S-EPMC5538065 | biostudies-other
| S-EPMC7225557 | biostudies-literature
| S-EPMC2600357 | biostudies-literature
| S-EPMC7699960 | biostudies-literature
| S-EPMC4540947 | biostudies-literature
| S-EPMC7070436 | biostudies-literature
| S-EPMC522358 | biostudies-literature
| S-EPMC7271422 | biostudies-literature
| S-EPMC4877633 | biostudies-literature