Counter-on-chip for bacterial cell quantification, growth, and live-dead estimations.

ABSTRACT: Quantifying bacterial cell numbers is crucial for experimental assessment and reproducibility, but the current technologies have limitations. The commonly used colony forming units (CFU) method causes a time delay in determining the actual numbers. Manual microscope counts are often error-prone for submicron bacteria. Automated systems are costly, require specialized knowledge, and are erroneous when counting smaller bacteria. In this study, we took a different approach by constructing three sequential generations (G1, G2, and G3) of counter-on-chip that accurately and timely count small particles and/or bacterial cells. We employed 2-photon polymerization (2PP) fabrication technology; and optimized the printing and molding process to produce high-quality, reproducible, accurate, and effic

SUBMITTER: Rahman KMT 

PROVIDER: S-EPMC10774380 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature