Novel method for collecting hippocampal interstitial fluid extracellular vesicles (EV^ISF ) reveals sex-dependent changes in microglial EV proteome in response to Aβ pathology.

ABSTRACT: Brain-derived extracellular vesicles (EVs) play an active role in Alzheimer's disease (AD), relaying important physiological information about their host tissues. The internal cargo of EVs is protected from degradation, making EVs attractive AD biomarkers. However, it is unclear how circulating EVs relate to EVs isolated from disease-vulnerable brain regions. We developed a novel method for collecting EVs from the hippocampal interstitial fluid (ISF) of live mice. EVs (EV^ISF ) were isolated via ultracentrifugation and characterized by nanoparticle tracking analysis, immunogold labelling, and flow cytometry. Mass spectrometry and proteomic analyses were performed on EV^ISF cargo. EV^ISF were 40-150 nm in size and expressed CD63, CD9, and CD81. Using a model of

SUBMITTER: Pait MC 

PROVIDER: S-EPMC10774707 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature