Transcriptomics and Spatial Proteomics for Discovery and Validation of Missing Proteins in the Human Ovary.

ABSTRACT: Efforts to understand the complexities of human biology encompass multidimensional aspects, with proteins emerging as crucial components. However, studying the human ovary introduces unique challenges due to its complex dynamics and changes over a lifetime, varied cellular composition, and limited sample access. Here, four new RNA-seq samples of ovarian cortex spanning ages of 7 to 32 were sequenced and added to the existing data in the Human Protein Atlas (HPA) database www.proteinatlas.org, opening the doors to unique possibilities for exploration of oocyte-specific proteins. Based on transcriptomics analysis of the four new tissue samples representing both prepubertal girls and women of fertile age, we selected 20 protein candidates that lacked previous evidence at the protein level, so

SUBMITTER: Mear L 

PROVIDER: S-EPMC10775140 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature